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KMID : 0985420070290020247
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Laboratory Medicine and Quality Assurance
2007 Volume.29 No. 2 p.247 ~ p.252
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Comparison of Various Methods for Detection of Methicillin-Resistant Staphylococcus aureus
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Lee Kyung
Park Kyoung-Un
Song Jung-Han
Song Sang-Hoon
Kim Eui-Chong
Heo Se-Ran
Choi Soon-He
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Abstract
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Background: Cefoxitin, a cephamycin-type antibiotic, is known to be superior to oxacillin in predicting the presence of mecA gene because it serves as a very potent inducer of mecA regulatory system. We used a cefoxitin disk diffusion methods for detection of methicillin-resistant Staphylococcus aureus (MRSA), and compared it with the conventional methods.
Methods: For 50 MRSA and 50 methicillin susceptible S. aureus confirmed by mecA and femA gene PCR, oxacillin, cefoxitin, and moxalactam disk diffusion methods, oxacillin and cefoxitin E-tests, Vitek 2 and Microscan Walkaway antibiotics susceptibility tests, and PBP2a latex agglutination test were performed. The sensitivity and specificity of each method were evaluated.
Results: The sensitivities of oxacillin disk diffusion method and E-test were 96%. The sensitivities of cefoxitin and moxalactam disk diffusion method, cefoxitin E-test, Vitek 2, Microscan Walkaway, PBP2a latex agglutination test were 100%. The specificities were 100% for all the methods used.
Conclusions: It may be considered that both the cefoxitin- and moxalactam disk diffusion methods are effective and excellent screening methods for the detection of MRSA in clinical laboratory routinely.
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KEYWORD
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MRSA, Cefoxitin, Moxalactam, Oxacillin, Disk diffusion methods
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